NanoMedical Systems is launching its new product platform, the nHance™ Peptide Capture System (PCS) to revolutionize peptide quantification. The nHance™ PCS 96-Well Plate features a thin film layer at the bottom of each well. The thin-film structure is composed of a nano-sized pore network tailored to the size of peptides. Peptides enter pores but larger proteins and other molecules remain in solution. Agnostic to any bioanalytical approach (LCMS, ELISA, etc.), this sample cleanup and peptide enrichment solution provides access to analytes in the range of 500-10,000 Dalton molecular weight in complex matrices The nHance™ PCS technology affords users a workflow under an hour with minimal sample pretreatment utilizing standard reagents that are commonly available in an LC-MS laboratory.
|Catalog Number||Description||Unit Size||Price|
|SKU: PCS1001-1||nHance™ PCS 96-Well Plate||1- Pack||$289.00|
|SKU: PCS1001-5||nHance™ PCS 96-Well Plate 5-Pack||5-Pack||$1,300.00|
The nHance™ Peptide Capture System* is a revolutionary, patented nanotechnology developed specifically for peptide enrichment and quantification eliminating many of the cumbersome steps associated with other approaches. The nanoporous coating at the heart of this system absorbs peptides within its nanopores based on size-exclusion, electrical charge, and hydrophobicity. However, unlike conventional sample preparation technologies on the market today, the peptides are first captured and then subsequently released after washing in a quick and seamless elution step for subsequent LC-MS analysis. It is truly a capture system for peptides and not a flow-through system based on retention time. As such, this approach allows sufficient time for both the size exclusion and electrostatic mechanisms of action to be fully utilized. Unwanted molecules and large debris are removed during the washing procedures. Peptides of interest are extracted for subsequent identification and quantification absent interference from the complex matrix.
|nHance™ Product Detail||Description|
|Operating Molecular Weight Range||Approximately 500-10,000 Daltons|
|nHance™ Coating Material||Proprietary nanoporous silica|
|Form Factor||Standard 96-Well Microtiter Plate, max volume 380mL|
|Suggested Operational Sample Volume||20-100µL|
Simplify Peptide Capture with nHance™ PCS – Peptide Capture System
- Designed specifically for peptides
- Standard 96-Well Plate Format
- Fast, simple and more cost-effective than SPE
- Label-free peptide enrichment
- Linear, accurate, and precise results
- Unsurpassed suppression of matrix effects
- No special equipment required (e.g. vacuum manifolds)
The nHance™ PCS – Peptide Capture System is a revolutionary, patented nanotechnology developed specifically for peptide quantification eliminating many of the cumbersome steps associated with other approaches. When comparing nHance™ PCS vs Solid Phase Extraction, the nHance™ PCS technology provides superior peptide extraction. The proprietary nanoporous coating featured at the bottom of each well absorbs peptides within its nanopores based on size‐exclusion, charge, and hydrophobicity. However, unlike conventional sample preparation technologies on the market today, the peptides are easily released in a quick and seamless elution step for subsequent liquid chromatography-mass spectrometry (LC-MS) analysis.
The nHance™ PCS product line allows for significant flexibility at the bench level to work with a wide range of peptides based on the following attributes:
- Molecular Weight (MW)
- Isoelectric point (pI)
Simple But Powerful
Unlike other approaches to sample preparation for liquid chromatography-mass spectrometry (LC-MS) analysis, the nHance™ PCS requires minimal sample pretreatment and eliminates the need for peripheral equipment such as centrifuges and vacuum manifolds. The longest step is the incubation step and you can be attending to other tasks while these samples are incubating in the wells. This relatively straightforward process achieves recovery exceeding 50% and variability below 10% with proven lab‐to‐lab transferability.